lactate enzymatic method

(A) propionic acid chloride Ethyl lactate conversion
The University of Tokyo, Kawasaki [6] and other studies of the use of purified L-2-halo acid dehalogenase and DL-2-halo acid dehalogenase were acting on the substrate L-2-chloro-propionic acid and DL- 2 - chloro acid, L-lactic acid obtained bittern off or D-lactic acid. L-2-halo acid dehalogenase catalyzed L-2-chloropropionic acid, and DL-2-halo acid dehalogenase catalyzed both L-2-chloropropionic acid, but also the catalytic L-2-chloropropyl generate the corresponding optically active acid, catalytic conversion simultaneous configuration.
(2) enzymatic conversion of pyruvate
Lactate dehydrogenase from the highest activity of Lactobacillus DSM20196 confusion cells obtained in D-lactic acid buffered lactic acid, a non-optically active pyruvate as substrate D-lactic acid can be obtained.
Industrial production of lactic acid fermentation method and the method is mainly synthesis. Because fermentation process is simple, abundant raw materials, develop and become more mature earlier lactic acid production methods, lactic acid production accounts for about 70 or more, but long cycle, only batch or semi-continuous production and quality of domestic non-lactic fermentation to international standards. Chemical method can achieve large-scale continuous production of lactic acid and lactic acid synthesis has also been the U.S. Food and Drug Administration (FDA) approval, but the raw materials are generally toxic, does not meet the requirements of green chemistry. Enzymatic process is complex, and its industrial application needs further study.